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层析柱法分离纯化多糖，可以一次完成。依据纯化的目的不同，可在过柱前脱色脱蛋白。脱蛋白后，吸收峰的值明显下降。
The separation and purification of polysaccharides by column chromatography can be completed at one time. Depending on the purpose of the purification, deproteinization can be performed before the column is decolorized. After deproteination, the absorption peak value decreased significantly.