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目的:研究左氧氟沙星与黄连中成分联合用药对多重耐药大肠埃希菌的体外抑制作用。方法:选择从临床标本中检出的多重耐药大肠埃希菌,分别测定黄连提取物、盐酸小檗碱、盐酸巴马汀及左氧氟沙星对多重耐药大肠埃希菌最小抑菌浓度(MIC),采用微量肉汤棋盘稀释法分别测定黄连提取物、盐酸小檗碱、盐酸巴马汀与左氧氟沙星的体外联合抑菌作用。结果:实验结果表明黄连提取物、盐酸小檗碱、盐酸巴马汀与左氧氟沙星联合用药后对大部分耐药菌(90.9%)均表现为协同或相加作用,其中盐酸巴马汀与左氧氟沙星的联合应用最有效。结论:盐酸巴马汀、盐酸小檗碱或黄连提取物分别与左氧氟沙星联合对多重耐药大肠埃希菌都有较好的体外抗菌作用,临床应用价值高,值得进一步研究。
Objective: To study the inhibitory effect of combination of levofloxacin and rhizoma coptidis on multidrug-resistant Escherichia coli in vitro. Methods: The multi-drug resistant Escherichia coli isolated from clinical specimens were selected to determine the MICs of Coptis Chinensis Franch, Berberine Hydrochloride, Palmatine Hydrochloride and Levofloxacin against multidrug-resistant Escherichia coli. , Respectively. The in vitro combined antibacterial activity of Coptis chinensis Franch, Berberine hydrochloride, Palmatine Hydrochloride and Levofloxacin were determined by microtiter plate chessboard dilution method. Results: The results showed that the combination of berberine hydrochloride, berberine hydrochloride and levofloxacin showed a synergistic or additive effect on most drug-resistant bacteria (90.9%). The combination of palmatine hydrochloride and levofloxacin Joint application of the most effective. Conclusion: Palmatine hydrochloride, berberine hydrochloride or berberine extract, respectively, combined with levofloxacin against multidrug-resistant Escherichia coli have good antibacterial activity in vitro, high clinical value, it is worth further study.