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采用小鼠卵母细胞体外培养、体外受精的方法研究不同产地 3种香烟的烟雾水溶物对小鼠卵母细胞成熟和受精能力的影响。结果显示 3种香烟烟雾水溶物对卵母细胞生发泡破裂都没有影响 ,但可以显著地抑制卵母细胞第一极体的释放、降低卵母细胞的体外受精率和体外存活率。结果提示 ,香烟烟雾中影响生殖作用的主要成分是其中的水溶性成分 ,它们可以破坏卵母细胞的成熟、降低卵细胞的受精能力 ,对生殖细胞和生育能力有一定的毒性作用 ;不同产地、不同品牌香烟烟雾水溶物毒性作用相似。
In vitro culture and in vitro fertilization of mouse oocytes were used to study the effects of aerosolized water-soluble substances from three kinds of cigarettes of different origins on mouse oocyte maturation and fertilization. The results showed that all the three kinds of cigarette smoke water soluble substances had no effect on the rupture of oocysts, but significantly inhibited the release of the first polar body of oocytes and the oocyte in vitro fertilization rate and in vitro survival rate. The results suggest that the main component affecting the reproductive effects of cigarette smoke is one of the water-soluble components, which can destroy the oocyte maturation, reduce the fertilization ability of the egg cells and have certain toxic effects on the germ cells and fertility; different origin, different Brand cigarette smoke water soluble toxic similar effects.