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目的 :为控制美洛昔康的含量和有关物质 ,建立了离子对高效液相色谱测定法。方法 :色谱柱为Alltech公司AlltimaODS柱 (2 5 0mm× 4 6mm) ,流动相 :乙腈 -含 0 2 5 %辛烷磺酸钠的 0 1mol·L- 1 磷酸二氢钠 (磷酸调至pH 2 5 ) (6 5∶35 ) ,流速 :1mL·min- 1 ,检测波长分别为 35 5nm (含量测定 )和 2 71nm (有关物质测定 )。结果 :美洛昔康在 9～ 110 μg·mL- 1 范围内呈良好的线性关系 ,r=0 9998。结论 :本文改进的离子对高效液相色谱法为测定美洛昔康及其制剂的含量和有关物质提供了可靠基础。
Objective: To control the content of meloxicam and related substances, established by ion-pair high performance liquid chromatography. Methods: Alltech column (250 mm × 4 6 mm) was equipped with a mobile phase of acetonitrile 0 1 mol·L -1 sodium dihydrogen phosphate containing 0 2 5% sodium octane sulfonate (pH adjusted to pH 2 5) (6:35:35), flow rate: 1mL · min-1, the detection wavelength was 35 5nm (content determination) and 2 71nm (related substances determination). Results: Meloxicam showed a good linearity in the range of 9-110 μg · mL-1, r = 0 9998. Conclusion: The improved ion-pair HPLC method provides a reliable basis for the determination of meloxicam and its preparations and related substances.