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目的:研究MGb1-Ag在人体各系统和器官的正常组织和肿瘤中的表达和分布。观察其在胃癌中表达的临床意义。方法:(1)免疫组织化学检测MGb1-Ag在人体各正常组织和常见肿瘤、正常胃粘膜、慢性胃炎、肠上皮化生、不典型增生、胃癌及淋巴结转移灶中的表达和分布,观察胃癌特异性抗原MGb1-Ag在全身各组织器官中的分布,统计分析其在胃癌中表达与临床病理参数之间的关系;(2)激光共聚焦检测MGb1-Ag在胃癌细胞中的定位。结果:MGb1-Ag仅在少数正常组织中有表达,在常见肿瘤中,主要在胃癌、结肠癌、食管癌、胰腺癌、前列腺癌和部分肝癌、肺癌中阳性表达,而在其它肿瘤中表达较少或几乎不表达。MGb1-Ag在胃癌中的表达与胃癌的分化、分期和淋巴结转移密切相关。MGb1-Ag定位于胃癌细胞系SGC-7901的细胞膜上。结论:MGb1-Ag是胃肠道特异性抗原,在胃癌中的表达与胃癌的分化和转移相关,是胃癌新的候选分子标志物。
OBJECTIVE: To study the expression and distribution of MGb1-Ag in normal tissues and tumors of various human systems and organs. To observe its clinical significance in gastric cancer. Methods: (1) Immunohistochemistry was used to detect the expression and distribution of MGb1-Ag in all kinds of normal tissues, common tumors, normal gastric mucosa, chronic gastritis, intestinal metaplasia, dysplasia, gastric cancer and lymph node metastasis. The distribution of specific antigen MGb1-Ag in various tissues and organs of the body was analyzed. The relationship between the expression of MGb1-Ag and clinicopathological parameters was statistically analyzed. (2) The localization of MGb1-Ag in gastric cancer cells was detected by confocal laser scanning microscopy. Results: MGb1-Ag was expressed only in a few normal tissues. It was mainly expressed in gastric cancer, colon cancer, esophageal cancer, pancreatic cancer, prostate cancer and some liver cancer and lung cancer, while in other tumors Few or almost no expression. The expression of MGb1-Ag in gastric cancer is closely related to the differentiation, staging and lymph node metastasis of gastric cancer. MGb1-Ag is localized on the cell membrane of gastric cancer cell line SGC-7901. Conclusion: MGb1-Ag is a gastrointestinal specific antigen, which is related to the differentiation and metastasis of gastric cancer. It is a new candidate molecular marker of gastric cancer.