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AIM:To study the change of intracellular calcium-magnesiumATPase (Ca~(2+)-Mg~(2+)-ATPase) activity in pancreas,liver andkidney tissues of rats with acute pancreatitis (AP),and toinvestigate the effects of Qingyitang (QYT) (Decoction forclearing the pancreas) and tetrandrine (Tet) and vitamin E(VitE) on the activity of Ca~(2+)-Mg~(2+)-ATPase.METHODS:One hundred and five Sprague-Dawley ratswere randomly divided into:normal control group,AP group,treatment group with QYI (1 ml/100 g) or Tet (0.4 ml/100 g)or VitE (100 mg/kg).AP model was prepared by a retrogradeinjection of sodium taurocholate into the pancreatic duct.Tissues of pancreas,liver and kidney of the animals weretaken at 1 h,5 h,10 h respectively after AP induction,andthe activity of Ca~(2+)-Mg~(2+)-ATPase was studied using enzyme-histochemistry staining.Meanwhile,the expression of Ca~(2+)-Mg~(2+)-ATPase of the tissues was studied by RT-PCR.RESULTS:The results showed that the positive rate ofCa~(2+)-Mg~(2+)-ATPase in AP group (8.3%,25%,29.2%) waslower than that in normal control group (100%) in all tissues(P<0.01),the positive rate of Ca~(2+)-Mg~(2+)-ATPase in treatmentgroup with QYT (58.3%,83.3%,83.3%),Tet (50.0%,70.8%,75.0%) and VitE (54.2%,75.0%,79.2%) was higherthan that in AP group (8.3%,25.0%,29.2%) in all tissues(P<0.01).RT-PCR results demonstrated that in treatmentgroups Ca~(2+)-Mg~(2+)-ATPase gene expression in pancreastissue was higher than that in AP group at the observingtime points,and the expression at 5 h was higher than thatat 1 h.The expression of Ca~(2+)-Mg~(2+)-ATPase in liver tissuewas positive,but without significant difference betweendifferent groups.CONCLUSION:The activity and expression of intracellularCa~(2+)-Mg~(2+)-ATPase decreased in rats with AP,suggesting thatCa~(2+)-Mg~(2+)-ATPase may contribute to the occurrence anddevelopment of cellular calcium overload in AP.QYT,Tetand VitE can increase the activity and expression of Ca~(2+)-Mg~(2+)-ATPase and may relieve intracellular calcium overloadto protect the tissue and cells from injuries.
AIM: To study the change of intracellular calcium-magnesium ATPase activity in pancreas, liver andkidney tissues of rats with acute pancreatitis (AP), and to investigate the effects of Qingyitang (QYT) Decoction forclearing the pancreas and tetrandrine (Tet) and vitamin E (VitE) on the activity of Ca ~ (2 +) - Mg ~ (2 +) - ATPase.METHODS: One hundred and five Sprague-Dawley ratswere The AP model was prepared by a retrogradeinjection of sodium taurocholate (100 mg / kg) was randomly divided into normal control group, AP group, treatment group with QYI (1 ml / 100 g) or Tet into the pancreatic duct.Tissues of pancreas, liver and kidney of the animals weretaken at 1 h, 5 h, 10 h respectively after AP induction and the activity of Ca ~ (2 +) - Mg ~ (2 +) - ATPase was studied using enzyme-histochemistry staining. Meanwhile, the expression of Ca ~ (2 +) - Mg ~ (2 +) - ATPase of the tissues was studied by RT-PCR.RESULTS: The results showed that the positive rate ofCa ~ (2+ ) -Mg ~ (2 +) - ATPase in AP group (8 The positive rate of Ca ~ (2 +) - Mg ~ (2 +) - ATPase in (3%, 25%, 29.2%) waslower than that in normal control group Treatment groups with QYT (58.3%, 83.3%, 83.3%), Tet (50.0%, 70.8%, 75.0%) and VitE (54.2%, 75.0%, 79.2%) were higherthan that in AP group (8.3%, 25.0% 29.2%) in all tissues (P <0.01) .RT-PCR results perform that in treatmentgroups Ca ~ (2 +) - Mg ~ (2 +) - ATPase gene expression in pancreastissue was higher than that in AP group at the observingtime points , and the expression at 5 h was higher than that at 1 h. The expression of Ca ~ (2 +) - Mg ~ (2 +) - ATPase in liver tissue was positive, but without significant difference betweendifferent groups. CONCLUSION: The activity and expression suggesting that Ca ~ (2 +) - Mg ~ (2 +) - ATPase may contribute to the occurrence and development of cellular calcium overload in AP .QYT, Tet and VitE can increase the activity and expression of Ca ~ (2 +) - Mg ~ (2 +) - ATPase and may relieve intracellular calcium overloadto protect the tissue and cells from injuries.