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作者研制了适用于在自动生化仪上测定血清LDH活性的LD-P紫外监测法试剂盒.实验室研究证明,自制试剂盒在Impact 400型自动生化仪上,与西德BM公司,美国Gilford公司同类试剂盒对比测定结果一致,呈非常显著相关.酶促反应的延迟时间短(<20s),血清LDH活性测定线性范围宽,可达1000u/L(37℃),批内变异系数1.1～4.7%.在4～8℃条件下贮存,本试剂盒可稳定7个月,重溶试剂可稳定7天.81名健康成人血清LDH活性测定结果为318.3±61.9u/L(37℃).
The author has developed LD-P UV monitoring kit suitable for measuring serum LDH activity on automatic biochemical analyzer.Laboratory studies have shown that homemade kits are compatible with West Germany BM, Gilford, USA Compared with the same kit, the results of the assay were consistent and significant. The enzymatic reaction had a short delay time (<20s) and the linear range of LDH activity was as high as 1000u / L (37 ℃). The intra-assay coefficient of variation was 1.1 ~ 4.7 %. Store at 4 ~ 8 ℃ for 7 months and re-dissolve the reagent for 7 days. The serum LDH activity of the 81 healthy adults was 318.3 ± 61.9u / L (37 ℃).