论文部分内容阅读
通过RT-PCR的方法分离了花青素合成途径上的关键结构基因片段:CHS、CHI、F3H、F3′H和DFR。在白色系、红色系、紫色系和蓝色系瓜叶菊舌状花中,采用半定量RT-PCR的方法分析CHS、CHI、F3H、F3′H、DFR、F3′5′H、3MaT这7个结构基因在花不同发育阶段的表达模式。结果表明:在白色花中不存在CHS的表达;在红色花中检测到F3′H基因的高丰度表达,但没有检测到F3′5′H的转录本;在蓝色花中没有F3′H表达的信号,但在花序开放的第Ⅰ阶段有较强的F3′5′H的表达信号;而在紫色花中可同时检测到F3′H和F3′5′H的转录本。此外,瓜叶菊花青素合成相关结构基因在花序开放初期高丰度表达,随后逐渐降低,在第Ⅳ阶段表达量再次升高,而在花序开放末期表达量极低或没有表达信号。
The key structural gene segments of anthocyanin synthesis pathway were isolated by RT-PCR: CHS, CHI, F3H, F3’H and DFR. CHS, CHI, F3H, F3’H, DFR, F3’5’H and 3MaT were analyzed by semi-quantitative RT-PCR in white, red, purple and blue squashes. The expression pattern of different structural genes in different developmental stages of flower. The results showed that there was no CHS expression in white flowers; high abundance expression of F3’H gene was detected in red flowers but no F3’5’H transcript was detected; there was no F3 ’ H, but there is a strong F3’5’H signal in the first stage of inflorescence, while the transcripts of F3’H and F3’5’H can be detected in purple flowers at the same time. In addition, the structure-related genes of anthocyanin synthesis in melon were highly abundant in the early stage of inflorescence, and then decreased gradually. In stage IV, the expression of anthocyanin increased again, while the expression was very low or no signal was expressed at the late stage of inflorescence.