论文部分内容阅读
目前我国生产的苏芸金杆菌(简称B、t)制剂,主要有粉剂和乳剂两种剂型。其质量标准主要以含活孢量为根据,活芽孢数在100亿/毫升(克)以上,菌剂含活孢量越大,其毒力也就越高,质量也就越好。下面介绍平板培养简易测定活孢子数的具体方法。 1.无菌水的准备。把一支装有90毫升水的三角瓶和9支装有9毫升水的试管加棉塞后经高压蒸气灭菌,备用。 2.准备直径为6—10cm的无菌培养皿10套,1毫升无菌吸管9支,10毫升无菌吸管1支。 3.取10毫升(克)菌剂样品,加入上述准备好的盛有90毫升无菌水三角瓶中,摇动制成10~(-1)菌悬液,置于75℃水浴锅中热处理15分钟(杀死一部分营养体)。
At present, China’s production of Bacillus thuringiensis (referred to as B, t) preparations, mainly two formulations of powder and emulsion. The quality standards are mainly based on the amount of living spores, the number of viable spores in 10 billion / ml (grams) above, the greater the amount of living bacteria containing agents, the higher their virulence, the better the quality. The following describes the specific method of plate culture simple determination of the number of live spores. 1. Sterile water preparation. Add a 90 mL flask and 9 tubes filled with 9 mL of water to a tampon and autoclave and set aside. 2. Prepare 10 sets of sterile Petri dishes 6-10cm in diameter, 9x1mL sterile tubes, and 1 mL sterile tubes. 3. Take 10 ml (g) of mycotoxin sample and add to the above prepared 90 ml sterile water in a flask, shake to make a 10-1 suspension and place in a 75 ° C water bath for heat treatment 15 Minutes (to kill part of the nutrition body).