论文部分内容阅读
目的:探讨益气化瘀方对产后子宫复旧不全模型大鼠肿瘤坏死因子α(TNF-α)、白介素-1(IL-1)、6-酮-前列腺素F1α(6-K-PGF1α)、血栓素B2(TXB2)的影响。方法:将72只雌鼠随机分为空白组、模型组、产复康组及益气化瘀方低、中、高剂量组(4.68,9.36,18.72 g·kg-1)。除空白组外,其余各组均造成子宫复旧不全模型,并于造模后第1～7天分别ig给药。采用双抗体夹心酶联免疫吸附法(ELISA)测定TNF-α值;采用免疫组化法检测IL-1含量;采用放免药盒测试法测定6-K-PGF1α和TXB2的含量。结果:与空白组相比,模型组IL-1平均灰度、TXB2显著降低(P<0.01),其余各指标升高(P<0.05,P<0.01)。与模型组相比,产妇康组6-K-PGF1降低无统计学意义,其余各指标变化差异性显著(P<0.05,P<0.01)。益气化瘀方中剂量组IL-1平均灰度值升高、IL-1面密度值及TNF-α值降低有统计学意义(P<0.05,P<0.01);益气化瘀方高剂量组各指标变化均有统计学意义(P<0.05,P<0.01)。与产妇康组相比,益气化瘀方高剂量组降低6-K-PGF1水平的作用较显著。而益气化瘀方各剂量组间比较,益气化瘀方高剂量组能更好调节PGI2/TXA2比值。结论:益气化瘀方能降低炎症因子TNF-α,IL-1水平,有抗炎作用;能调节PGI2/TXA2比值,促进PGI2/TXA2之间的动态平衡,使血止而不留瘀,从而发挥治疗产后子宫复旧不全之功效。益气化瘀方高剂量组疗效显著。
Objective: To investigate the effects of Yiqi Huayu decoction on the expression of TNF-α, IL-1, 6-K-PGF1α, Effect of thromboxane B2 (TXB2). Methods: Seventy-two female rats were randomly divided into blank group, model group, producing Fukangkang group and low, medium and high dose Yiqi Huayu Decoction group (4.68,9.36,18.72 g · kg-1). In addition to the blank group, the rest of the groups were caused by incomplete uterine involution model, and in the first 7 days after modeling ig administration. Tumor necrosis factor-α (TNF-α) levels were measured by enzyme-linked immunosorbent assay (ELISA). The levels of IL-1 were detected by immunohistochemistry. The levels of 6-K-PGF1α and TXB2 were determined by radioimmunoassay. Results: Compared with the blank group, the average gray level of IL-1 and TXB2 in the model group were significantly decreased (P <0.01), and the other indexes were increased (P <0.05, P <0.01). Compared with the model group, 6-K-PGF1 in maternal serum was not significantly reduced (P <0.05, P <0.01). The mean gray value of IL-1 in Yiqi Huayu Recipe middle dose group was increased, the surface density of IL-1 and TNF-α decreased significantly (P <0.05, P <0.01) The indexes in the dose group had statistical significance (P <0.05, P <0.01). Compared with maternal health group, Yiqi Huayu Fang high-dose group reduced 6-K-PGF1 level more significant. The Yiqi Huayu prescription dose comparison, Yiqi Huayu Fang high dose group can better adjust PGI2 / TXA2 ratio. Conclusion: Yiqi Huayu Recipe can reduce the levels of inflammatory cytokines TNF-α and IL-1 and has anti-inflammatory effects. It can regulate PGI2 / TXA2 ratio and promote the dynamic balance between PGI2 / TXA2, To play the treatment of postpartum uterine involution incomplete effect. Yiqi Huayu Fang high dose group significant effect.