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目的 :建立固相萃取结合高效液相色谱法对人体血浆中阿替洛尔含量进行检测 ,为阿替洛尔的体内血药浓度监测和人体药代动力学研究提供测定方法。方法 :血样经固相萃取法处理 ,高效液相色谱荧光检测法测定 ,外标法定量。结果 :本方法血样处理简单、杂质少、干扰小。高、中、低浓度的绝对回收率平均为 81.4 % ,阿替洛尔体内治疗浓度范围内 ,检测线性关系良好 ,回归方程为A =3.4 0× 10 3 C +9.92× 10 3 ,r=0 .9993。日内误差与日间误差的RSD均低于 8% ,精密度和准确度都能满足药动学研究及血药浓度监测的要求。结论 :固相萃取结合高效液相色谱法测定血浆中阿替洛尔浓度重复性好、干扰小 ,是一种较为理想的测量方法。
OBJECTIVE: To establish a solid phase extraction coupled with high performance liquid chromatography (HPLC) for the determination of atenolol in human plasma and to provide a method for the determination of atenolol in vivo blood concentration and pharmacokinetics. Methods: The blood samples were processed by solid-phase extraction, high performance liquid chromatography (HPLC) fluorescence detection method and external standard method. Results: The method of blood sample processing is simple, less impurities, interference. The average recoveries of high, medium and low concentrations were 81.4% on average, and the linear relationship was good for the concentration of atenolol in vivo. The regression equation was A = 3.4 × 10 3 C + 9.92 × 10 3, r = 0 .9993. Intra-day error and daytime error RSD were less than 8%, precision and accuracy can meet the pharmacokinetic studies and plasma concentration monitoring requirements. Conclusion: Solid phase extraction combined with high performance liquid chromatography in the determination of plasma concentration of atenolol repeatability, small interference, is an ideal measurement method.