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为了明确关中地区越冬茬番茄黄化曲叶病毒病发生和流行规律,通过分析该病发生与番茄品种、定植期及传播介体烟粉虱之间的关系,并采用PCR技术对田间病原进行分子鉴定。结果表明,番茄黄化曲叶病毒病在8月中下旬至11月上中旬开始侵染,翌年3月中下旬发生再侵染,秋季病情减轻;烟粉虱种群数量与病害发生程度呈线性正相关;不同番茄品种对番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的抗性差异显著,其中大番茄品种布鲁尼1288和DRW7728,小番茄品种千禧和美红对该病表现为免疫;分子检测结果表明,4个样品中均扩增出543 bp的特异片段,与NCBI数据库Gen Bank的TYLCV序列(登录号为GU084381、KC138544.1、KC138543.1和JX456642.1)的相似性达99%。研究表明,关中地区番茄病毒病为番茄黄化曲叶病毒病,番茄品种、定植期及烟粉虱发生动态是影响该病发生的主要因素。
In order to clarify the occurrence and epidemic of tomato yellow leaf curl leaf virus over the winter in Guanzhong area, we analyzed the relationship between the disease occurrence and occurrence of tomato leafhopper, Identification. The results showed that tomato yellow curl leaf virus disease started to infiltrate from late August to mid-November and re-infested in the middle and late March of the following year, reducing the disease in autumn. The population of Bemisia tabaci was positively correlated with the degree of disease occurrence . The resistance of different tomato varieties to Tomato yellow leaf curl virus (TYLCV) was significantly different. Among them, the large tomato varieties Bruni 1288 and DRW7728 and the mini tomato varieties Millenium and Meihong were immunized against the disease The results of molecular analysis showed that all the 4 samples amplified a 543 bp fragment with similarity to the TYLCV sequences of GenBank in NCBI database (accession numbers GU084381, KC138544.1, KC138543.1 and JX456642.1) 99%. Studies have shown that tomato virus disease in Guanzhong area is tomato yellow leaf curl virus disease, and the occurrence of tomato species, colonization and whitefly is the main factor affecting the occurrence of the disease.