论文部分内容阅读
采用茎尖分生组织培养技术 ,获得了大蒜 (AlliumsativumL .)的无病毒试管苗。通过基本培养基和激素配比试验 ,筛选出最佳的培养基组成 ,进行脱病毒苗的快速繁殖。结果表明 :诱导愈伤组织的最适培养基为 :MS +BA0 .2mg/L +NAA 0 .5mg/L ,月生长率达 12 70倍 ;诱导丛生芽的最适培养基为 :B5+BA 0 .5mg/L +IAA 0 .2mg/L ,丛生芽繁殖系数高达 2 5 5倍 ,技术上达到了快速繁殖规模生产的要求。用电子显微镜反复进行了病毒检测 ,其中有 5个样品脱病毒彻底 ,将作为今后提供无病毒优质种苗的原种苗。
Using shoot tip meristem culture technique, virus-free in vitro seedlings of Allium sativum L. were obtained. Through the basic medium and hormone ratio test, the best medium composition was screened for rapid propagation of virus-free vaccine. The results showed that the optimum medium for inducing callus was MS + BA0.2mg / L + NAA0.5mg / L, with a monthly growth rate of 1270 times. The optimal culture medium for inducing shoots was B5 + BA 0 .5mg / L + IAA 0 .2mg / L, the multiplication coefficient of clustered shoots was up to 25.5 times, which reached the requirement of rapid propagation and scale production technically. Repeated virus scanning with electron microscopy, of which 5 samples thoroughly virus-free, will serve as the future of seedless seedlings of high-quality virus-free seedlings.