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目的:探讨湘西通道侗族高血压病(essential hypertension,EH)人群血管紧张素转化酶(angiotensin converting enzyme,ACE)基因第16内含子中的插入/缺失(insertion/deletion,I/D)多态性与血清生化指标之间的关系。方法:采用聚合酶链反应检测了93例湘西通道侗族高血压病患者和99名正常对照的ACE基因16内含子I/D多态性,血清K+、Na+、TC、HDL-C、TG采用自动生化分析仪检测。结果:①正常对照组和高血压病组ACE-I/D多态性II、ID、DD基因型频率分别为47.5%、42.4%、10.1%和40.9%、41.9%、17.2%,两组基因型频率分布无统计学差异(χ2=2.26,P=0.32);②正常对照组和/或高血压病组II、ID、DD基因型与血清K+、Na+、TC、HDL-C、TG指标之间差异无统计学意义;③合计分析发现,携带ACE-I/D多态性DD基因型人群血清总胆固醇(TC)要高于ID和II基因型(F检验P=0.05)。结论:湘西通道侗族人群ACE基因16内含子I/D多态性与当地高血压病之间可能无关,但当地人群中携带ACE-I/D多态性DD基因型者可能与血清总胆固醇(TC)的升高有一定的关联。
Objective: To investigate the polymorphism of insertion / deletion (I / D) in intron 16 of angiotensin converting enzyme (ACE) gene in Dong people with essential hypertension (EH) Sex and serum biochemical indicators of the relationship. Methods: ACE gene 16 intron I / D polymorphism was detected by polymerase chain reaction in 93 cases of Dong nationality hypertension and 99 normal controls in Xiangxi Dong people. Serum K +, Na +, TC, HDL-C and TG were detected by polymerase chain reaction Automatic biochemical analyzer testing. Results: ① The frequencies of ACE-I / D polymorphism II, ID and DD genotypes in normal control group and hypertension group were 47.5%, 42.4%, 10.1% and 40.9%, 41.9% and 17.2% (Χ2 = 2.26, P = 0.32). ② The genotypes of II, ID, DD in normal control group and / or hypertension group were significantly different from those in serum K +, Na +, TC, HDL-C and TG There was no significant difference between the two groups (P> 0.05). ③Total analysis showed that the serum total cholesterol (TC) of DD genotypes carrying ACE-I / D polymorphism was higher than that of ID and II genotypes. Conclusion: There is no correlation between ACE gene 16 intron I polymorphism and local hypertension in Dong population in western Hunan Province. However, the DD genotype carrying ACE-I / D polymorphism in local population may be associated with serum total cholesterol (TC) increased a certain correlation.