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目的:对4株幽门螺杆菌(Helicobacter pylori,Hp)中hp0521基因编码的细胞毒素相关基因2(cytotoxin-associated gene 2,Cag2)蛋白从生物信息学角度进行基本理化性质、信号肽及功能的预测分析。方法:用PCR技术克隆测序获得4株Hp菌株的hp0521基因序列,生物信息学工具分析其编码Cag2蛋白的基本理化性质、有无信号肽及蛋白指纹图谱。结果:成功克隆测序4株Hp菌株的hp0521基因序列,提交基因库后获得相应登录号;hp0521基因编码的Cag2蛋白氨基酸数目和相对分子质量大小不同,理论等电点偏碱性,为稳定亲水性蛋白,不存在信号肽;Cag2蛋白指纹图谱分析存在DNA拓扑异构酶Ⅰ、IL-3细胞因子、抗增殖蛋白BTG1家族、介导细胞壁延伸的蛋白、卤酸脱卤酶/环氧水解酶、调节染色体凝集功能RCC1家族等蛋白标签。结论:Hp中hp0521基因编码的Cag2蛋白可能介导细胞壁延伸和调节染色体凝集,参与致病信号通路的传导,可能具有DNA拓扑异构酶Ⅰ、卤酸脱卤酶/环氧水解酶等相应酶活性。
OBJECTIVE: To predict the basic physico-chemical properties, signal peptides and functions of 4 genes encoding hp0521 gene encoding Helicobacter pylori (Hp) from the perspective of bioinformatics analysis. Methods: The hp0521 gene sequences of four Hp strains were cloned and sequenced by PCR. The bioinformatics tools were used to analyze the basic physical and chemical properties of the Cag2 protein. The signal peptide and protein fingerprinting were also analyzed. Results: The sequence of hp0521 gene was successfully cloned and sequenced. The gene sequence of hp0521 gene was cloned and sequenced. The amino acid number and relative molecular mass of Cag2 protein encoded by hp0521 gene were different. The theoretical isoelectric point was more alkaline, Protein, no signal peptide present; Cag2 protein fingerprinting analysis of the presence of DNA topoisomerase I, IL-3 cytokines, anti-proliferating protein BTG1 family, proteins that mediate cell wall elongation, halo acid dehalogenase / , Regulate the chromosome agglutination function RCC1 family protein tags. CONCLUSION: Cag2 protein encoded by hp0521 gene in Hp may mediate cell wall extension and regulate chromosomal agglutination, which may be involved in the pathogenicity of signal transduction pathways. It may have DNA topoisomerase I, halogen acid dehalogenase / epoxy hydrolase and other enzymes active.