目的探讨培养细胞的最佳染色方法。方法通过比较细胞直接染色、细胞涂片染色、琼脂石蜡双重包埋法、电镜包埋染色四种染色方法为本实验室建立一套最佳的细胞染色方法。结果细胞直接染色和细胞涂片染色法显示出的细胞比较直观,结构清晰,但常出现细胞不集中、细胞重叠等缺陷。而用琼脂石蜡双重包埋法,结果显示细胞分布均匀、集中,便于染色与观察。结论琼脂石蜡双重包埋法是一种简便易行、重复性好的细胞切片染色方法。 Objective To investigate the best method of cell culture. Methods By comparing the cell direct staining, cell smear staining, agar paraffin double embedding method, electron microscopy embedding staining four staining methods for the laboratory to establish a set of the best cell staining method. Results Cell direct staining and cell smear staining showed that the cells were more intuitive and the structure was clear. However, the defects of cell concentration and cell overlap often appeared. However, with double embedding agar paraffin, the results showed that the cells were evenly distributed, concentrated, easy to stain and observe. Conclusions The double embedding method of agar wax is a simple and reproducible method of staining cell sections.