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从石油污染土壤中筛选得到一株石油降解菌SYBS01,鉴定为假单胞菌(Pseudomonas sp.).通过单因素实验和正交实验进行石油降解条件优化,结果为35℃、170 r·min-,酵母浸粉15 g· L-1,KH2PO40.5g·L-1,自然pH值.其中氮源为酵母浸粉时对菌SYBS01降解石油的影响最大.添加酵母浸粉后,4d石油降解率达到85.6％,分别为(NH4)2SO4、NH4NO3和尿素的6.7、7.8、2.6倍.进一步分析发现酵母浸粉作为氮源的同时也起到外加营养的作用.在最佳条件下,0.5～5 g·L-1的石油的降解均符合一级动力学模型,且石油浓度为3g·L-1和4g·L-1的石油降解半衰期仅为26 h.“,”A petroleum degrading bacterial strain SYBS01 was isolated from oil-contaminated soil,and was identified as Pseudomonas sp.Oil degradation conditions were compared with single-factor and orthogonal experimental design,with the results showing that the optimum conditions were 35 ℃,170 r · min-1,yeast extract 15 g · L-1,KH2PO4 0.5 g · L-1,and a natural pH value.Among these,the yeast extract had the greatest influence on the oil-degradation rate.The oil-degradation rate of SYBS01 increased to 85.6％ four days after yeast extract had been added to the system,and was 6.7,7.8,and 2.6 times higher than were (NH4)2SO4,NH4NO3,and CON2H4,respectively.It was shown that yeast extract not only acted as a source of nitrogen,but also provided extra nutrition.A first-order kinetic model could be fitted to the oil degradation for the entire concentration range in optimum conditions.Furthermore,the half-life was found to be 26 h for oil 3 to 4 g · L-1.