目的 探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)及受体Bek在骨巨细胞瘤的表达情况,探索骨巨细胞瘤的生物学行为规律与预后影响因素。方法 采用免疫组化方法、Mias-2000图像分析技术检测了147例骨巨细胞瘤(复发组56例,未复发组91例)石蜡标本中Bek/bFGF的表达情况,用流式细胞术分析其DNA含量与S期细胞百分数(S-phase fraction,SPF),结合肿瘤直径、浸润情况、手术方式、复发情况等相关临床病理资料,采用多元回归分析方法综合分析骨巨细胞瘤的预后影响因素。结果 bFGF、Bek在骨巨细胞瘤主要瘤细胞成分中广泛表达,阳性信号主要定位于瘤细胞胞浆;复发组瘤组织中bFGF、Bek均呈强阳性表达的异形性较大的单核、双核及三核基质细胞与4～8个核的多核巨细胞明显增多,而未复发组瘤组织中bFGF、Bek阴性或弱阳性表达的体积大核多(多达几十个)的多核巨细胞数目较多;复发组bFGF、Bek表达信号较未复发组明显增强,两组间bFGF、Bek表达的灰度值与阳性面积差异均有显著意义。复发组平均DNA指数(DNA Index,DI)为1.24,SPF为18.3％;未复发组平均DI值为1.05,SPF为11.7％;两组间DI值、SPF差异均有显著意义。在13个相关研究因素中,有7个因素与骨巨细胞瘤的预后显著相关,按影响强度由大到小依次为:手术方式? Objective To investigate the expression of basic fibroblast growth factor (bFGF) and its receptor Bek in giant cell tumor of bone, and to explore the biological behaviors and prognostic factors of giant cell tumor of bone. Methods Immunohistochemistry and Mias-2000 image analysis were used to detect the expression of Bek / bFGF in 147 cases of giant cell tumor of bone (recurrent group, non-recurrent group, 91 cases). Flow cytometry was used to analyze the expression of Bek / DNA content and S-phase fraction (S-phase fraction, SPF), tumor diameter, infiltration, surgical methods, recurrence and other relevant clinical and pathological data using multiple regression analysis of integrated prognosis of giant cell tumor of the factors. Results bFGF and Bek were widely expressed in the major tumor cells of giant cell tumor of bone. The positive signal mainly located in the cytoplasm of tumor cells. The bFGF and Bek in the tumor tissue of the recurrence group showed strongly positive heterotypic mononuclear and dual nuclei And multinucleate giant cells of trichome stromal cells and 4 ~ 8 nuclei increased significantly, while the number of multinuclear giant cells (up to several dozen) with large or bulky nuclei expressed in bFGF, Bek negative or weak positive cells While the expression of bFGF and Bek in the recurrence group was significantly higher than that in the non-recurrence group. There was significant difference between the gray value and the positive area of ​​bFGF and Bek expression in the two groups. The average DNA index (DI) of relapse group was 1.24 and the SPF was 18.3%. The average DI value of non-recurrence group was 1.05 and the SPF was 11.7%. There was significant difference in DI value and SPF between the two groups. In 13 related research factors, there are 7 factors that are significantly associated with the prognosis of giant cell tumor of bone, according to the intensity of descending order: surgery?