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为更好地利用抗TMV烤烟种质资源,提高烤烟抗TMV育种效率,对烤烟品系CV87、FC8、抗88的TMV抗性遗传规律及抗性来源进行了研究。利用抗病烤烟品系CV87、FC8、抗88分别与感病品种云烟87、中烟100配置杂交组合,构建F1、F2群体,并利用TMV-C菌株进行抗性鉴定;同时,设计N基因引物对参试烤烟品种(系)的基因组DNA进行PCR扩增。经抗性鉴定,CV87、FC8、抗88及F1群体对TMV免疫,云烟87、中烟100感TMV,卡方(χ2)检验证明F2群体抗感分离比为3∶1,符合显性单基因遗传;PCR结果表明,抗病品系CV87、FC8、抗88基因组内存在N基因序列,感病品种云烟87、中烟100基因组内未发现。本研究表明,CV87、FC8、抗88烤烟品系的TMV抗性来源于N基因。
In order to make better use of anti-TMV flue-cured tobacco germplasm resources and improve the anti-TMV breeding efficiency of flue-cured tobacco, the genetic laws of TMV resistance and the sources of resistance of CV87, FC8 and anti-88 in flue-cured tobacco were studied. The resistant hybrids CV87, FC8 and anti-88 were used to construct hybrid F1 and F2 populations with susceptible varieties Yunyan87 and Zhongyan100, respectively. The resistant strains were identified by TMV-C strain. At the same time, the N gene primer pair Genomic DNA of tobacco varieties (lines) tested for PCR amplification. The resistance of TMV and CV87, FC8, anti-88 and F1 populations were tested by resistance test, Yunfu87, TMV100 and chi-square test were used to test the resistance ratio of F2 population to 3: 1, in line with the dominant single gene The results of PCR showed that there were N gene sequences in the resistant strains CV87, FC8 and anti-88 genome. The susceptible varieties Yunyan87 and Zhongyan100 were not found. The present study shows that the TMV resistance of CV87, FC8, anti-88 flue-cured tobacco lines is derived from the N gene.