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作者等在Hawkcs(1982)及Herbrink(1982)所建立的抗原斑点试验(Antigen Spot Test简称AST)的基础上,发展了一种用酶(HRP)标记疟疾抗原检测疟疾抗体的方法,即酶标记抗原斑点试验(Enzyme La-belled Antigen Spot Test,简称ELAST),具有简便、稳定、可靠的效果。 感染食蟹猴疟原虫(plasmodium cynomolgi,简称Pc)的恒河猴,切脾,待疟原虫增殖至10％虫血水平左右,且以裂殖体期和大滋养体期为主时放血。用肝素抗凝,离心去血浆,反复洗血球,分出棕褐色感染
Based on the Antigen Spot Test (AST) established by Hawkcs (1982) and Herbrink (1982), the authors developed a method for detecting malaria antibodies by using enzyme-linked immunosorbent assay (HRP) Antigen spot test (Enzyme La-belled Antigen Spot Test, referred to as ELAST), with simple, stable and reliable results. Infection with cynomolgus macaques (plasmodium cynomolgi, referred to as Pc) rhesus, spleen, to be malaria parasite proliferation to about 10% of the worm blood level, and the schizonts and large nocturnal bleeding. Anticoagulant with heparin, centrifuged to plasma, washed blood cells repeatedly, the separation of tan infection