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目的探讨NDRG4甲基化检测在结直肠癌诊断中的价值。方法从84例结直肠癌手术切除癌组织、癌旁组织及其术前粪便、尿、血中提取DNA,应用巢式甲基化特异性PCR(nested methylation specific PCR,nMSP)检测结直肠癌粪便、尿、血中NDRG4基因甲基化水平。比较结直肠癌组织和粪便、尿、血中NDRG4甲基化检测在结直肠癌诊断中的敏感性和特异性。结果在84例结直肠癌中,癌组织NDRG4检出率为81.0%,癌旁组织检出率为8.3%,敏感性为81.0%,特异性为91.7%;结直肠癌中粪便NDRG4基因甲基化的敏感性和特异性在3个微量DNA中最高,尿次之。结论结直肠癌组织NDRG4基因甲基化异常发生率高;粪便和尿中NDRG4甲基化异常可作为结直肠癌早期诊断的肿瘤标志物。
Objective To investigate the value of NDRG4 methylation detection in the diagnosis of colorectal cancer. Methods DNA was extracted from 84 cases of colorectal cancer surgically resected from cancer tissue, para-cancerous tissue and its preoperative stool, urine and blood, and the colorectal cancer stool was detected by nested methylation specific PCR (nMSP) , Urine, blood NDRG4 gene methylation levels. To compare the sensitivity and specificity of NDRG4 methylation detection in colorectal cancer tissues and stool, urine and blood in the diagnosis of colorectal cancer. Results In 84 cases of colorectal cancer, the detection rate of NDRG4 was 81.0% in cancer tissues, 8.3% in paracancerous tissues, the sensitivity was 81.0% and the specificity was 91.7%. In colorectal cancer, the NDRG4 gene methyl The sensitivity and specificity of the three trace DNA in the highest, followed by urine. Conclusions The abnormal methylation of NDRG4 gene in colorectal cancer tissues is high. Abnormal methylation of NDRG4 in feces and urine can be used as a tumor marker for early diagnosis of colorectal cancer.