目前,医院内发生的耐甲氧西林金黄色葡萄球菌(MRSA)的感染增多,鉴定MRSA流行株的方法有MRSA特异性噬菌体分型等多种,但噬菌体的多变性妨碍准确分型。近年来,运用针对MRSA的mecA基因序列和存于原核生物基因组中可变DNA序列的多聚酶链反应(PCR)进行MRSA分型有所发展,提供了一种相对快速、简单的方法。本文将传统的MRSA噬菌体分型法与mecA基因的PCR法进行比较,同时还比较了一种采用肠杆菌科中存在的DNA重复序列人工引 Currently, there is an increase in the incidence of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals, and the identification of MRSA-specific strains is based on MRSA-specific phage typing, but the variability in phage precludes accurate typing. In recent years, MRSA typing has been developed using the mecA gene sequence directed against MRSA and the polymerase chain reaction (PCR) of variable DNA sequences in the genome of prokaryotes, providing a relatively quick and easy method. In this paper, the traditional MRSA phage typing method and mecA gene PCR method were compared, but also compared using a sequence of Enterobacteriaceae DNA repetitive sequence artificial