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目的:检测大鼠在妊娠中晚期不同的二氧化碳(CO2)气腹时间和气腹压力下血液中血管性血友病因子(vWF)的含量变化。方法:采用固相夹心法酶联免疫吸附实验(ELISA)检测妊娠中、晚期大鼠在不同CO2气腹压力及时间内大鼠血液中vWF的含量变化。结果:(1)成功建立妊娠大鼠CO2气腹模型;(2)妊娠晚期胎盘血管损伤加重,但与CO2气腹无关。低CO2气腹压力即使长时间气腹对胎盘血管影响不大,高CO2气腹压力短时间气腹也对胎盘血管影响不大,但高CO2气腹压力长时间会对胎盘血管有严重影响(P<0.05)。结论:长时间高气腹压力可能导致胎盘血管的损伤,但继续妊娠对胎盘功能的影响尚不明确,妊娠期腹腔镜手术建议在妊娠中期进行,采用低气腹压力,手术时间在2h内为宜。
OBJECTIVE: To detect changes of vWF in blood during pneumoperitoneum (pneumoperitoneum) and pneumoperitoneum (pneumoperitoneum) in different stages of pregnancy. Methods: The contents of vWF in the blood of rats in the second trimester and the second trimester in different CO2 pneumoperitoneum pressure and time were detected by solid phase sandwich enzyme-linked immunosorbent assay (ELISA). Results: (1) CO2 pneumoperitoneum was successfully established in pregnant rats; (2) placental vascular injury was aggravated in late pregnancy, but not with CO2 pneumoperitoneum. Even if pneumoperitoneum has little effect on placental blood vessels for a long time, pneumoperitoneum with high CO2 pneumoperitoneum has little effect on placental blood vessels, but high CO2 pneumoperitoneum pressure will have a serious impact on placental blood vessels for a long time ( P <0.05). Conclusion: The pneumoperitoneum pressure may cause placental vascular injury after a long period of time, but the effect of continuing pregnancy on placental function is not clear. Laparoscopic surgery in pregnancy is recommended during the second trimester of pregnancy. Using low pneumoperitoneum pressure, the operation time is within 2h should.