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地下水源中真菌大量繁殖会产生嗅味,引发毒性反应以及产生大量肉眼可见的絮状物,严重影响供水水质.以地下水源中3种优势真菌木霉属、青霉属、枝孢属为研究对象,以氯为消毒剂,通过研究真菌孢子胞内物质泄漏,胞外三磷酸腺苷(ATP),脱氧核糖核酸(DNA)和蛋白质增加及孢子形态变化,探明了氯灭活3种真菌孢子的效能与机制.结果表明,氯灭活真菌孢子符合一级动力学,满足Chick模型,3种真菌的耐氯性为:木霉属>青霉属>枝孢属,真菌孢子尺寸越大,亲水性越强,灭活效果越好.氯灭活后,胞内物质泄漏,胞外特征物质(ATP、DNA、蛋白质)显著增加;氯对细胞表面破坏严重,灭活后真菌孢子凹陷,表面褶皱.综上所述,氯首先作用于孢子表面,降低孢子的可培养性,进一步作用使细胞的通透性屏障受损,导致胞内特征物质释放,以致孢子死亡.
In the groundwater source, the multiplication of fungi can produce odor, trigger the toxic reaction and produce a large amount of macroscopic flocs, seriously affecting the water quality of the water supply.Three dominant fungi Trichoderma, Penicillium and Cladosporium in groundwater are studied In this study, chlorine was used as disinfectant to study the effects of chlorine inactivation on the spores of three kinds of fungi by studying the intracellular leakage of fungal spores, the extracellular ATP, DNA and protein, and spore morphological changes The results showed that the chlorinated inactivated spores conformed to the first order kinetics and satisfied the Chick model. The chlorine tolerance of the three fungi was: Trichoderma> Penicillium> Cladosporium. The larger the fungal spore size, The stronger the sex, the better the inactivation effect.The leakage of intracellular substance and the extracellular characteristic substances (ATP, DNA, protein) were significantly increased after chlorine inactivation; the destruction of cell surface by chlorine was serious, In summary, chlorine first acts on the surface of the spores, reducing the culturability of the spores, further impairing the permeability barrier of the cells, resulting in the release of intracellular characteristic substances that cause the spores to die.