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目的了解并比较槲皮素、黄芪甲苷对体外培养的缺氧心肌细胞的保护作用,寻找其作用的量效关系。方法将SD乳鼠心肌细胞分成单纯缺氧组(A组)、缺氧+100 mg/L槲皮素组(B组)、缺氧+50 mg/L槲皮素组(C组)、缺氧+25 mg/L槲皮素组(D组)、缺氧+50.0 mg/L黄芪甲苷组(E组)、缺氧+25.0 mg/L黄芪甲苷组(F组)、缺氧+12.5 mg/L黄芪甲苷组(G组)、缺氧+10 mg/L维生素E组(H组)。各组细胞原代培养后先加入相应浓度的槲皮素、黄芪甲苷、维生素E,再行缺氧处理12 h(A组培养后直接行缺氧处理)。检测各组心肌细胞活力和乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、活性氧(ROS,只检测A、C、F、H组)值。结果B～G组与A组比较,LDH、MDA、ROS(C、F组)值下降,心肌细胞活力、SOD值上升,作用普遍优于H组。在同等高、中、低浓度下,加入黄芪甲苷组的心肌细胞活力和LDH水平总体优于加入槲皮素组(如C、F组的心肌细胞活力为0.454±0.018、0.471±0.017,LDH为2800±9、2312±52),但两组MDA、SOD和ROS值比较,差异无统计学意义(C、F组的ROS为16.0±5.3、22.4±8.7,P>0.05)。结论黄芪甲苷、槲皮素能有效保护缺氧心肌细胞,减轻损伤程度,其作用优于维生素E。黄芪甲苷的保护作用较槲皮素更好,但两者减轻细胞脂质过氧化损伤的作用差异不明显。
Objective To understand and compare the protective effects of quercetin and astragaloside on cultured hypoxic cardiomyocytes in vitro and to find the dose-effect relationship. Methods SD rat cardiomyocytes were divided into single hypoxia group (A group), hypoxia +100 mg/L quercetin group (B group), hypoxia +50 mg/L quercetin group (C group), and lacunas. Oxygen +25 mg/L Quercetin (Group D), Hypoxia +50.0 mg/L Astragaloside (Group E), Hypoxia +25.0 mg/L Astragaloside (Group F), Hypoxia + 12.5 mg/L astragaloside group (G group), hypoxia +10 mg/L vitamin E group (H group). After the primary culture of each group of cells, the corresponding concentrations of quercetin, astragaloside IV, and vitamin E were added, followed by an anoxic treatment for 12 hours (after the culture in group A, the animals were subjected to hypoxia treatment directly). Cardiomyocyte viability and lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and reactive oxygen species (ROS, only A, C, F, and H groups) were measured in each group. Results Compared with group A, the values of LDH, MDA, ROS (C, F) decreased, and the cardiomyocyte viability and SOD value increased in group B-G compared with group A. The effect was generally better than that in group H. At the same high, medium, and low concentrations, cardiomyocyte viability and LDH levels in the astragaloside group were generally superior to those in the quercetin group (eg, the cardiomyocyte viability in the C and F groups was 0.454±0.018, 0.471±0.017, LDH. It was 2800±9, 2312±52), but there was no significant difference in MDA, SOD and ROS values between the two groups (ROS in C and F groups was 16.0±5.3, 22.4±8.7, P>0.05). Conclusion Astragaloside IV and quercetin can effectively protect the hypoxic cardiomyocytes and reduce the degree of damage. Its effect is better than that of vitamin E. The protective effect of astragaloside was better than that of quercetin, but there was no significant difference between the two in reducing lipid peroxidation.