笋秀夜蛾雄蛾对性信息素化合物的电生理和行为反应

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利用气质联用仪对笋秀夜蛾雌蛾性腺体粗提物成分进行了分析比对,通过触角电位(EAG)试验研究了笋秀夜蛾雄成虫对性信息素组分的电生理反应,以Y形嗅觉仪进行了室内和林间的行为生测.结果表明:雌蛾性腺体粗提物中顺11-十六碳乙酸酯(Z11-16:Ac)和顺11-十六碳烯醇(Z11-16:OH)为笋秀夜蛾性信息素活性组分,性腺粗提物、两种化合物及其混合物均能激发雄蛾明显的EAG反应,且EAG反应值随性信息素组分浓度增加而增加.Z11-16:Ac和Z11-16:OH按57∶43配置成的二元混合物所激发的EAG反应强于同等剂量下的单一组分.室内的行为生测和林间诱捕试验结果与EAG反应基本一致,Z11-16:Ac+Z11-16:OH(57∶43)混合物对雄蛾引诱力最强,在林间单诱捕器(诱芯浓度为104ng·μL-1)的平均诱捕量达到(48.5±6.7)头. The GC-MS was used to analyze the components of the gynogenetic extracts of female moths, and the electrophysiological responses of sex pheromone components to male adults of Spodoptera exigua The results showed that the content of cis-11-hexadecetoacetate (Z11-16: Ac) and cis-11-hexadecanoic acid The alkenol (Z11-16: OH) is the active component of the sex pheromone of Gossypium hirsutum. The gonadal crude extracts, both compounds and their mixtures can stimulate the obvious EAG responses of male moths. The EAG response values ​​vary with the sex Z11-16: Ac and Z11-16: OH in 57:43 configured binary mixture excited EAG response stronger than the same dose of single component.Indoor behavior bioassay and The results of inter-forest trapping experiment were basically the same as those of EAG reaction. The mixture of Z11-16: Ac + Z11-16: OH (57:43) had the highest attraction to male moths. The single trap (concentration of 104ng · μL -1), the average trapping amount reached (48.5 ± 6.7) head.
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