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目的建立SYBR Green Ⅰ实时荧光定量PCR技术在法医物证检验中的应用。方法应用SYBR Green Ⅰ实时荧光定量PCR技术对各种生物检材进行定量分析,并在此基础上进一步分析STR。结果得到了实验的各种生物检材的准确定量。结论 SYBRSYBR Green Ⅰ实时荧光定量PCR技术是一种高效且廉价的检测基因拷贝数的方法,具有法医学应用价值。
Objective To establish the SYBR Green Ⅰ real - time fluorescence quantitative PCR technique for forensic evidence test. Methods SYBR Green Ⅰ real-time fluorescence quantitative PCR was used to quantitatively analyze various biopsy samples. Based on the results, STR was further analyzed. The results obtained the accurate quantification of various biological samples tested. Conclusion SYBRSYBR Green Ⅰ real-time fluorescence quantitative PCR is an efficient and cheap method to detect gene copy number, which has the value of forensic science.