论文部分内容阅读
目的：建立一种新缺氧动物模型，为保健品抗缺氧实验提供新筛选方法。方法：选用初成年雄性昆明种小鼠，随机分为对照组和缺氧组。对照组动物在常压环境条件下喂养，缺氧组动物在常压缺氧舱中喂养。舱内通入缺氧气体（氮：空气为1：1的混合气，氧浓度为10％±1％），连续20天。分别于缺氧5天、10天、15天、20天分批处死缺氧组动物，第20无处死对照组动物。取动物肺组织作病理检查。选择计数小于100微米肌性血管（MA）、部分肌性血管（PMA）、非肌性血管（NMA）的肺血管数及三者的构成比。结果：随缺氧时间延长，缺氧组MA、PMA的比例较对照组明显增大（P＜0．01），而NMA的比例较对照组明显减少。缺氧15天和20天三种血管的比例越于稳定。结论：在氧气浓度为10％的环境中连续缺氧15天至20天可形成较为理想的慢性缺氧性小鼠肺血管结构重建模型。
OBJECTIVE: To establish a new animal model of anoxia to provide a new screening method for hypnosis prevention of health products. Methods: Primary male Kunming mice were randomly divided into control group and hypoxia group. Animals in the control group were fed under ambient atmospheric conditions and animals in the hypoxic group were fed in an anaerobic tank. Into the cabin of oxygen-deficient gas (nitrogen: air 1: 1 mixture of oxygen concentration of 10% ± 1%) for 20 days. Rats in hypoxic group were sacrificed 5 days, 10 days, 15 days and 20 days hypoxia, respectively. Take animal lung tissue for pathological examination. Select the count less than 100 microns muscular (MA), partial muscular (PMA), non-muscular (NMA) of the pulmonary blood vessels and the composition ratio of the three. Results: With the prolongation of hypoxia time, the proportion of MA and PMA in hypoxia group was significantly higher than that in control group (P <0.01), but the proportion of NMA in hypoxia group was significantly lower than that in control group. Oxygen 15 days and 20 days the proportion of the three blood vessels more stable. CONCLUSION: Continuous hypoxia for 15 days to 20 days in an oxygen concentration of 10% can form an ideal pulmonary vascular remodeling model in chronic hypoxic mice.