Virtual organization uses information technology to achieve a closer integration and better management of business relationships between internal and external parties. There are many emerging issues in virtual organization and one of them is knowledge sharing. Knowledge sharing that is the core of knowledge management plays a key role in virtual organization. However, game theoretic exploration about knowledge sharing mechanism based on virtual organization is seldom published. In this study, knowledge sharing mechanism in virtual organization enterprise is explored using game theories from two aspects based on the features of knowledge sharing in virtual organization enterprise. Finally, the critical model of knowledge sharing mechanism in virtual organization also is presented based on the perspective of game analysis. Virtual organization uses information technology to achieve a closer integration and better management of business relationships between internal and external parties. There are many emerging issues in virtual organization and one of them is knowledge sharing. Knowledge sharing that is the core of knowledge management plays a key role in virtual organization. However, game theoretic exploration about knowledge sharing mechanism based on virtual organization is seldom published. In this study, knowledge sharing mechanism in virtual organization enterprise is explored using game theories from two aspects based on the features of knowledge sharing in virtual Finally, the critical model of knowledge sharing mechanism in virtual organization also is presented based on the perspective of game analysis.