目的探讨胶质瘤U251细胞中MGMT表达与卡莫司汀(BCNU)和替莫唑胺(TMZ)化疗耐药的关系。方法细胞生长情况用MTT进行评估,细胞周期采用流式细胞仪检测,MGMT表达用western-blot检测。结果 U251细胞对BCNU和TMZ存在耐药现象。在化疗过程中,处于G0/G1期细胞比例明显增加,MGMT表达无显著变化。结论利用BCNU和TMZ对U251进行化疗过程中,不能通过消耗MGMT,来达到逆转耐药现象的目的 。 Objective To investigate the relationship between the expression of MGMT in glioma U251 cells and the chemoresistance of BCNU and TMZ. Methods Cell growth was assessed by MTT. Cell cycle was detected by flow cytometry. MGMT expression was detected by western-blot. Results U251 cells were resistant to BCNU and TMZ. In the course of chemotherapy, the proportion of cells in G0 / G1 phase increased significantly, while the expression of MGMT did not change significantly. Conclusions The use of BCNU and TMZ in the chemotherapy of U251 can not achieve the goal of reversing the drug resistance by consuming MGMT.