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目的探讨新疆哈萨克族食管癌(Esophageal Carcinoma,EC)患者LRRFIP1基因甲基化状态及其与食管癌发生及预后之间的关系。方法采用亚硫酸氢盐修饰联合限制性内切酶分析法(combined bisulfite restriction analysis,COBRA)检测哈萨克族共28例食管癌及癌旁组织标本LRRFIP1基因启动子区甲基化状况,建立Cox回归模型分析临床病理指标及LRRFIP1基因甲基化水平等因素与预后的关系。结果 28例哈族食管癌患者癌组织和癌旁正常组织的LRRFIP1基因启动子甲基化率分别为71.43%和46.43%,差异有统计学意义(P<0.05)。预后因素分析表明肿瘤浸润程度、TNM分期、淋巴结转移是影响哈族食管癌预后的独立危险因素。结论 LRRFIP1基因启动子区甲基化状态与新疆哈族食管癌的发生有密切的关系,但可能与食管癌预后无关。
Objective To explore the relationship between the methylation status of LRRFIP1 gene and the occurrence and prognosis of esophageal cancer in Kazakh Esophageal Carcinoma (EC) patients. Methods The bisulfite restriction analysis (COBRA) was used to detect the methylation status of LRRFIP1 gene promoter in 28 cases of Kazakh patients with esophageal cancer and adjacent tissues. The Cox regression model was established. The relationship between clinical pathological parameters and methylation of LRRFIP1 gene and prognosis was analyzed. Results The methylation rates of the LRRFIP1 gene promoter in cancer tissues and normal tissues adjacent to tumor in 28 cases of Kazakh esophageal cancer were 71.43% and 46.43%, respectively, and the difference was statistically significant (P<0.05). Prognostic factors analysis showed that tumor infiltration, TNM stage, and lymph node metastasis were independent risk factors for the prognosis of Kazakh esophageal cancer. Conclusion The methylation status of LRRFIP1 gene promoter region is closely related to the occurrence of esophageal cancer in Xinjiang Kazakh ethnic group, but it may not be related to the prognosis of esophageal cancer.