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目的：在胰岛素的干预下，观察ＭＡＰＫ反义寡核苷酸（ＯＤＮｓ）对人血管平滑肌细胞（ＶＳＭＣ）增殖及ＰＫＣα表达的影响。方法：３ＨＴｄＲ掺入法检测ＶＳＭＣ增殖，逆转录ＰＣＲ、免疫组织化学法检测ＰＫＣα表达。结果：反义ＯＤＮｓ 处理的细胞可显著抑制胰岛素诱导的ＶＳＭＣ的ＤＮＡ合成，ＯＤＮｓ 的上述作用与降低ＶＳＭＣ内ＰＫＣα基因表达有关。结论：胰岛素刺激人ＶＳＭＣ增殖可被ＭＡＰＫ反义寡核苷酸所抑制，可能存在有关胰岛素ＰＫＣＭＡＰＫ激活途径
OBJECTIVE: To observe the effect of ODNs on proliferation and PKCα expression of human vascular smooth muscle cells (VSMCs) under the intervention of insulin. Methods: The proliferation of VSMCs was detected by 3H-TdR incorporation, and the expression of PKCα was detected by immunohistochemistry. RESULTS: Antisense ODNs treatment significantly inhibited insulin-induced VSMC DNA synthesis. The above effects of ODNs were related to the decrease of PKCα gene expression in VSMCs. Conclusion: Insulin-stimulated proliferation of human VSMCs can be inhibited by MAPK antisense oligonucleotides, and there may exist insulin-PKC MAPK activation pathway