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观察脑缺血再灌流后海马本部与齿状回 c GMP反应细胞的差异。钳夹沙土鼠的双侧颈总动脉制造脑缺血模型 ,应用免疫荧光组织化学方法。结果表明 :海马本部 c GMP合成增加 ,c GMP主要分布于 CA1 区的放射层及腔隙分子层 ,双重免疫荧光反应证实多数 c GMP阳性细胞是星形胶质细胞。齿状回与海马本部不同 ,较对照组增加了一些中小圆形的 c GMP阳性细胞 ,分布在齿状回各层。本实验结果揭示 ,短暂性前脑缺血再灌流可增加海马本部 c GMP的合成 ,多数 c GMP阳性细胞是星形胶质细胞。意味着星形胶质细胞在脑缺血再灌流早期扮演着重要角色。
To observe the differences of hippocampus and dentate gyrus cGMP cells after cerebral ischemia and reperfusion. The bilateral common carotid arteries of the gerbils were used to make the cerebral ischemia model and the immunofluorescence histochemistry method was used. The results showed that: c GMP synthesis increased in the hippocampus, c GMP mainly distributed in the radioactive layer and lacunar molecular layer in the CA1 area, double immunofluorescence confirmed that most of the c GMP positive cells were astrocytes. Dentate gyrus and hippocampus different from the control group increased the number of small and medium circular c GMP positive cells, distributed in the dentate gyrus layers. The results of this study revealed that transient forebrain ischemia-reperfusion can increase the cGMP synthesis in the hippocampus of the hippocampus and most of the GMP-positive cells are astrocytes. Which means that astrocytes play an important role in the early stage of cerebral ischemia and reperfusion.