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目的:研究溴氰菊酯(DM)对大鼠外周血淋巴细胞DNA的损伤作用及对肝脏功能的影响。方法:32只雌性Wistar大鼠随机分成4组,染毒剂量分别为0,3.125,6.250,12.500mg/kg,连续灌胃染毒10天。DNA损伤采用单细胞凝胶电泳(彗星实验)进行评价,并测定丙氨酸氨基转移酶(ALT)以反映肝功能变化。结果:染毒组大鼠外周淋巴细胞的尾DNA%(Tail DNA%)、尾矩(Tail Moment)和Olive尾矩(Olive Tail Moment)均高于对照组(P<0.05),差别有统计学意义。各染毒组与对照组的肝功能差异均无统计学意义。结论:DM可导致外周血淋巴细胞DNA损伤。
Objective: To study the effect of deltamethrin on the DNA damage of rat peripheral blood lymphocytes and its effect on liver function. Methods: Thirty-two female Wistar rats were randomly divided into 4 groups. The doses were 0, 3.125, 6.250, 12.500 mg / kg, respectively. DNA damage was evaluated by single cell gel electrophoresis (comet assay) and alanine aminotransferase (ALT) was measured to reflect changes in liver function. Results: Tail DNA%, Tail Moment and Olive Tail Moment of the peripheral lymphocytes in the exposed group were significantly higher than those in the control group (P <0.05), the differences were statistically significant significance. There was no significant difference in liver function among the infected and control groups. Conclusion: DM can cause DNA damage in peripheral blood lymphocytes.