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目的：探讨ＡＦＰｍＲＮＡ在人肝细胞癌中的表现规律。方法：用ＲＴ－ＮｅｓｔｅｄＰＣＲ技术检测３０例肝癌组织及癌旁组织ＡＦＰｍＲＮＡ；放免法检测其血清ＡＦＰ。结果：２８例癌组织ＡＦＰｍＲＮＡ阳性（２８／３０），２例癌旁组织ＡＦＰｍＲＮＡ阳性；９例血清ＡＦＰ阴性者有７例癌组织ＡＦＰｍＲＮＡ阳性；２例癌组织ＡＦＰｍＲＮＡ及血清ＡＦＰ均阴性。结论：人肝细胞癌ＡＦＰ基因表达调控形式不同，ＡＦＰｍＲＮＡ在早期诊断肝细胞癌中有一定价值。
Objective: To investigate the expression of AFP mRNA in human hepatocellular carcinoma. Methods: RT-Nested PCR was used to detect AFP mRNA in 30 cases of hepatocellular carcinoma and adjacent tissues. Serum AFP was detected by radioimmunoassay. Results: AFPmRNA was positive in 28 cases (28/30). AFPmRNA was positive in 2 paratumor tissues. AFPmRNA was positive in 7 cases of 9 AFP-negative patients. AFPmRNA and AFP were negative in 2 cases. Conclusion: The expression of AFP gene in human hepatocellular carcinoma is different. The AFPmRNA has a certain value in the early diagnosis of hepatocellular carcinoma.