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ＲＴＰＣＲ结合图像分析技术进行微量核酸样品的相对定量和绝对定量，具有较高的灵敏度和精确度，通过非竞争ＰＣＲ求微量样品的相对量，竞争ＰＣＲ求样品的绝对含量。因此，ＲＴＰＣＲ结合图像分析技术适合于ｍＲＮＡ水平上基因表达的分析。
RT PCR combined with image analysis techniques for the relative quantification and absolute quantification of trace nucleic acid samples with high sensitivity and accuracy, the relative amount of trace samples obtained by non-competitive PCR, competitive PCR to determine the absolute content of the sample. Therefore, RT PCR combined with image analysis technology is suitable for gene expression analysis at the mRNA level.