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据国内丝虫病工作者报道,我国不同地区马来丝虫病患者,不论在临床表现、微丝蚴的周期性夜现高峰时间和主要传播媒介蚊种等方面都有不同程度的差异,为探索我国不同地区周期型马来丝虫是否存在种下分型的问题,本文采用扫描电镜对四川乐山(L)、贵州独山(D)和福建建阳(J)地区马来丝虫成虫的趣微结构进行观察比较。一、材料与方法分别从实验感染三地区马来丝虫长爪沙鼠Meriones unguiculatus的腹腔,获取L株(4♀♀8♂♂)、D株(4♀♀4♂♂)和J株(4♀♀2♂♂)成虫,用pH7.4的PBS充分洗净,2.5％戊二醛和1 锇酸作前固定和后固定,然后各用PBS清洗数次,经酒精梯度脱水,以醋酸异戊酯置换,置
According to reports of domestic filariasis workers, the incidence of malaria filariasis in different areas of China varies in varying degrees in terms of clinical manifestations, peak nighttime recurrence of microfilariae, and major vectors of mosquitoes To explore whether there is a problem of sub-genotyping of periodic malayian filariasis in different regions of our country. In this paper, scanning electron microscopy (SEM) was used to analyze the genetic diversity of adult malayian filariasis in Leshan (L), Dushan (D), Guizhou and Jianyang (J) Interest microstructure observation and comparison. MATERIALS AND METHODS MATERIALS AND METHODS: Four strains of M (4♀♀8♂♂), D (4♀♀4♂♂) and J (4♀♀4♂♂) were obtained from the abdominal cavity of Meriones unguiculatus, 4 ♀♀ 2 ♂ ♂) adults, washed well with pH 7.4 PBS, 2.5% glutaraldehyde and 1 osmic acid for pre-fixed and post-fixed, and then washed with PBS several times, dehydrated by alcohol gradient to acetic acid Isoamyl replacement, set