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目的探讨胰岛素样生长因子结合蛋白-3(insulin like growth factor binding protein-3,IGFBP-3)在白藜芦醇(resveratrol,Res)抑制脑胶质瘤C6细胞增殖中的作用。方法将脑胶质细胞和脑胶质瘤C6细胞分为脑胶质细胞组、Res+脑胶质细胞组、脑胶质瘤C6细胞组及Res+脑胶质瘤C6细胞组,采用MTT和Western blot法分别检测Res对各组细胞增殖及细胞中IGFBP-3和ERK蛋白表达水平的影响。将脑胶质瘤C6细胞分为空白对照组、Res组、si RNA-IGFBP-3组和Res+si RNA-IGFBP-3组,采用MTT法、流式细胞术、Transwell试验及Western blot法分别检测Res联合si RNA-IGFBP-3对脑胶质瘤C6细胞增殖、凋亡、侵袭能力及细胞中IGFBP-3和ERK蛋白表达水平的影响。结果各组细胞培养48 h后,Res+脑胶质细胞组与脑胶质细胞组的细胞增殖抑制率及细胞中IGFBP-3和ERK蛋白的表达水平均无明显变化(P>0.05);Res+脑胶质瘤C6细胞组与脑胶质瘤C6细胞组比较,细胞增殖抑制率和细胞中ERK蛋白的表达水平均明显降低(P<0.05),而IGFBP-3蛋白的表达水平明显升高(P<0.05)。与空白对照组比较,Res组细胞增殖抑制率、凋亡率、IGFBP-3蛋白表达水平明显升高(P<0.05),侵袭能力、ERK蛋白表达水平明显降低(P<0.05);而si RNA-IGFBP-3组和Res+si RNA-IGFBP-3组细胞增殖抑制率、凋亡率、IGFBP-3蛋白表达水平明显降低(P<0.05),侵袭能力、ERK蛋白表达水平明显升高(P<0.05)。结论 Res可能是通过增强IGFBP-3的表达来抑制ERK信号通路的活性,进而发挥促进脑胶质瘤细胞凋亡的作用。
Objective To investigate the effect of insulin like growth factor binding protein-3 (IGFBP-3) on the proliferation of glioma C6 cells induced by resveratrol (Res). Methods C6 glioma and glioma cells were divided into glioma group, Res + glial cell group, C6 glioma group and C6 glioma cell group. MTT and Western blot Method were used to detect the effect of Res on the cell proliferation and the expression of IGFBP-3 and ERK protein in each group. Glioma C6 cells were divided into blank control group, Res group, si RNA-IGFBP-3 group and Res + si RNA-IGFBP-3 group. MTT assay, flow cytometry, The effect of Res combined with si RNA-IGFBP-3 on the proliferation, apoptosis, invasion and the expression of IGFBP-3 and ERK in glioma C6 cells were detected. Results After 48 h of culture, the inhibitory rate of cell proliferation and the expression of IGFBP-3 and ERK protein in Res + brain glia cells and glial cells groups were not significantly different (P> 0.05). Res + brain Compared with glioma C6 glioma C6 glioma cell group, the inhibition rate of cell proliferation and the expression of ERK protein were significantly decreased (P <0.05), while the expression of IGFBP-3 protein was significantly increased (P <0.05). Compared with the blank control group, the cell proliferation inhibition rate, apoptosis rate and the expression of IGFBP-3 protein in Res group were significantly increased (P <0.05), and the invasion ability and ERK protein expression were significantly decreased (P <0.05) The proliferation inhibition rate, apoptosis rate and IGFBP-3 protein expression in IGFBP-3 group and Res + si RNA-IGFBP-3 group were significantly decreased (P <0.05), while the invasion ability and ERK protein expression were significantly increased <0.05). Conclusion Res may inhibit the activity of ERK signaling pathway by enhancing the expression of IGFBP-3, and then play a role in promoting glioma cell apoptosis.