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G蛋白偶联受体激酶(GRK)是G蛋白偶联受体(GPCR)信号通路的负性调节因子。近来的研究发现,GRK除了磷酸化G蛋白偶联受体使其脱敏外,还能与其他非受体底物结合,功能呈现多样性。GRK5是GRK家族成员之一,该研究探索了GRK5在细胞周期和有丝分裂中的作用,结果显示:在细胞内干扰GRK5的表达导致分裂中期的细胞数目增多和细胞凋亡。进一步的研究发现,干扰GRK5的表达导致有丝分裂中期的染色体不能正常排列到赤道板,而对分裂后期染色质分离以及胞质分裂没有影响。在细胞内干扰GRK蛋白家族的另一个成员GRK2对有丝分裂则没有明显影响。该研究提示GRK5是细胞有丝分裂的重要调控蛋白。
G protein-coupled receptor kinase (GRK) is a negative regulator of the G-protein coupled receptor (GPCR) signaling pathway. Recent studies have found that, in addition to GRK phosphorylated G protein-coupled receptor to desensitization, but also with other non-receptor substrate binding, showing diversity in function. GRK5, a member of the GRK family, explores the role of GRK5 in cell cycle and mitosis. The results show that intracellular interference with GRK5 leads to an increase in the number of cells and apoptosis in the metaphase. Further studies found that interrupting the expression of GRK5 resulted in metaphase chromosomes can not be normal to the equatorial plate, while the late division of chromatin separation and cytokinesis did not affect. GRK2, another member of the GRK family of proteins that interferes with the cell, has no apparent effect on mitosis. The study suggests that GRK5 is an important regulatory protein in mitosis.