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目的:探讨乌梅丸及其寒热配伍对于2型糖尿病(T2DM)大鼠外周组织AMP蛋白激酶[adenosine 5’-monophosphate(AMP)-activated protein kinase,AMPK]表达的影响。方法:采用喂养高脂饮食8周并尾静脉注射小剂量链脲佐菌素(STZ)的方法建立T2DM大鼠模型,乌梅丸及其寒热配伍组分别治疗8周。检测治疗前后大鼠体质量的变化、口服葡萄糖耐量试验(OGTT)、空腹血清胰岛素(FINS)、计算胰岛素抵抗指数(HOMA-IR),分别用Western Blot和RT-PCR法检测肝脏、骨骼肌、脂肪组织中AMPK蛋白和mRNA表达水平。结果:与正常组比较,模型组体质量增加、OGTT明显异常,FINS、HOMA-IR升高,肝脏、骨骼肌、脂肪组织中AMPK蛋白和mRNA表达水平均降低;与模型组比较,乌梅丸及其寒热配伍各组大鼠体质量和OGTT改善,FINS、HOMA-IR下降,肝脏、骨骼肌、脂肪组织中AMPK蛋白和mRNA表达水平明显升高。但乌梅丸组与其寒热配伍各组之间的差异不明显。结论:乌梅丸及其寒热配伍可有效提高肝脏、骨骼肌、脂肪组织AMPK的表达。
Objective: To investigate the effect of compatibility of Radix Umami Pill and its combination of chills and heat on the expression of AMP protein (AMP) in peripheral tissue of type 2 diabetes mellitus (T2DM) rats. Methods: T2DM rat model was established by feeding a high-fat diet for 8 weeks and injecting a small dose of streptozotocin (STZ) via the tail vein. The Wumei pill and its cold-heat compatibility group were treated for 8 weeks respectively. The changes of body weight, oral glucose tolerance test (OGTT), fasting serum insulin (FINS) and insulin resistance index (HOMA-IR) were measured before and after treatment. The levels of liver, skeletal muscle, Adipose tissue AMPK protein and mRNA expression levels. Results: Compared with the normal group, the body mass of the model group increased, OGTT was significantly abnormal, FINS and HOMA-IR increased, and the levels of AMPK protein and mRNA in the liver, skeletal muscle and adipose tissue were decreased. Compared with the model group, The body weight and OGTT of rats in each group were improved, the levels of FINS and HOMA-IR were decreased, and the levels of AMPK protein and mRNA in liver, skeletal muscle and adipose tissue were significantly increased. However, there is no obvious difference between Wume pill group and its cold and heat compatibility groups. Conclusion: Wumei pill and its compatibility with cold and heat can effectively improve the liver, skeletal muscle and adipose tissue AMPK expression.