论文部分内容阅读
目的探讨去甲斑蝥素对膀胱尿路上皮癌T24细胞生物学行为影响。方法用噻唑蓝法检测不同浓度去甲斑蝥素对T24细胞增殖的抑制作用;用Hoechst 33342染色法检测T24细胞凋亡率;Transwll实验检测去甲斑蝥素对T24细胞侵袭力抑制作用;用酶联免疫法检测T24细胞基质金属蛋白酶-2(MMP-2)及血管内皮生长因子(VEGF)表达水平。结果各剂量去甲斑蝥素均能明显抑制T24细胞生长,呈剂量依赖性(P<0.05);与空白对照组比较,去甲斑蝥素4、16μmol/L组T24细胞凋亡率[分别为(16.02±0.75)%、(28.28±1.17)%]明显增加,透膜细胞数[分别为(23.33±1.53)、(16.17±2.47)个]明显下降,差异均有统计学意义(P<0.05);与空白对照组比较,去甲斑蝥素4、16μmol/L组T24细胞MMP-2[分别为(5.04±1.02)、(3.02±1.15)]和VEGF[分别为(0.65±0.09)、(0.48±0.11)]表达水平明显降低,差异有统计学意义(P<0.05)。结论去甲斑蝥素可抑制T24细胞增殖,促进细胞凋亡,降低细胞侵袭能力,抑制细胞MMP-2及VEGF表达。
Objective To investigate the effect of norcantharidin on the biological behavior of bladder urothelial carcinoma T24 cells. Methods The inhibitory effect of different concentrations of norcantharidin on the proliferation of T24 cells was detected by thiazolyl blue method. The apoptosis rate of T24 cells was detected by Hoechst 33342 staining. The inhibitory effect of norcantharidin on invasiveness of T24 cells was detected by Transwll assay. Immunoassay was used to detect the expression of matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) in T24 cells. Results Norcantharidin significantly inhibited the growth of T24 cells in a dose-dependent manner (P <0.05). Compared with the blank control group, the apoptotic rates of T24 cells in norcantharidin 4 and 16 μmol / L groups were ( 16.02 ± 0.75)%, (28.28 ± 1.17)%] significantly increased, the number of transmembrane cells [(23.33 ± 1.53) and (16.17 ± 2.47) respectively] decreased significantly with statistical significance (P0.05) (5.04 ± 1.02, respectively, 3.02 ± 1.15) and VEGF [(0.65 ± 0.09) and (0.48, respectively, compared with the blank control group) ± 0.11)], the difference was statistically significant (P <0.05). Conclusion Norcantharidin can inhibit T24 cell proliferation, promote apoptosis, reduce cell invasion, and inhibit the expression of MMP-2 and VEGF.