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目的:探讨血管内皮生长因子(VEGF)和神经纤毛蛋白-2(Nrp-2)在大肠腺癌组织中的表达、相互关系及与临床病理的关系。方法:采用免疫组织化学SP法检测VEGF和Nrp-2在大肠腺癌及癌旁组织中的表达和分布。应用SPSS 16.0软件包对数据进行χ2检验和直线相关分析。结果:VEGF和Nrp-2在大肠腺癌组织中的表达率分别是:68.29%(28/41)和78.05%(32/41),明显高于癌旁组织中的表达率20%(2/10)和0%(0/10),差异有统计学意义(P<0.05)。VEGF和Nrp-2蛋白在大肠腺癌组织中的表达有明显的正相关性(r=0.837,P<0.05)。大肠腺癌组织中VEGF与Nrp-2的表达均与肿瘤大小、浸润深度及淋巴结转移等相关(P<0.05),而与肿瘤分化程度、部位、患者年龄和性别无关(P>0.05)。结论:Nrp-2可能与大肠腺癌组织中的血管及淋巴管形成相关,VEGF与Nrp-2协同在大肠腺癌的发展、浸润和转移过程中发挥着作用。
Objective: To investigate the expression of vascular endothelial growth factor (VEGF) and neuropilin-2 (Nrp-2) in colorectal adenocarcinoma and its relationship with clinical pathology. Methods: Immunohistochemical SP method was used to detect the expression and distribution of VEGF and Nrp-2 in colorectal adenocarcinoma and paracancerous tissues. The SPSS 16.0 software package was used for χ2 test and linear correlation analysis. Results The expression rates of VEGF and Nrp-2 in colorectal adenocarcinoma were 68.29% (28/41) and 78.05% (32/41), respectively, which were significantly higher than those in paracancerous tissues (20%, 2 / 10) and 0% (0/10), the difference was statistically significant (P <0.05). There was a significant positive correlation between VEGF and Nrp-2 protein expression in colorectal adenocarcinoma (r = 0.837, P <0.05). The expression of VEGF and Nrp-2 in colorectal adenocarcinoma was correlated with tumor size, depth of invasion and lymph node metastasis (P <0.05), but not with the degree of tumor differentiation, location, age and gender of patients (P> 0.05). Conclusion: Nrp-2 may be involved in the formation of blood vessels and lymphangiogenesis in colorectal adenocarcinoma. The synergistic effect of VEGF and Nrp-2 may play an important role in the development, invasion and metastasis of colorectal adenocarcinoma.