论文部分内容阅读
目的建立可靠的小肠黏膜微血管内皮糖萼显示方法,为探讨其生物学功能提供基础。方法取小鼠6只,分为对照组和心脏灌注组,每组3只。对小鼠行乙醚吸入麻醉,心脏灌注组使用梯度阿尔新蓝溶液心脏灌注固定后取空肠组织,对照组直接取空肠组织,然后按照常规方法对其黏膜层制备超薄切片,透射电镜观察。结果透射电镜观察显示,心脏灌注组小鼠肠黏膜微血管内皮细胞膜表面有明显的糖萼结构,呈不连续型和连续型两种形态,不连续性糖萼厚度为50～100nm,连续型糖萼厚度为20～30nm;而对照组未能观察到明显的糖萼结构。结论常规透射电镜样品处理方法,不能显示肠黏膜微血管内皮细胞的糖萼,而心脏灌注阿尔新蓝溶液进行前固定,能够较好地保存该结构,是一种较为可靠的研究方法。
Objective To establish a reliable method for revealing the calyx in the microvascular endothelium of the small intestine to provide a basis for exploring its biological function. Methods Six mice were divided into control group and cardiac perfusion group, three in each group. The mice were anesthetized with ether inhalation. The cardiac perfusion group was treated with gradient Alzheimer’s solution and perfused with jejunal tissue. The control group was given jejunal tissue directly. Then ultrathin sections of the mucosa were prepared by conventional methods and observed by transmission electron microscopy. Results Transmission electron microscopy showed that there were obvious glycocalyx structures on the surface of intestinal mucosa microvascular endothelial cells in cardiac perfusion group, which were discontinuous and continuous. The thickness of discontinuous glycocalyx was 50 ~ 100 nm. The continuous glycocalyx Thickness of 20 ~ 30nm; while the control group failed to observe obvious glycocalyx structure. Conclusion Conventional transmission electron microscopy sample processing method, can not show the glycocalyx of intestinal mucosal microvascular endothelial cells, and cardiac perfusion of alginate blue solution for pre-fixation, can better preserve the structure, is a more reliable research methods.