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目的研究新化合物二鼠李糖脂衍生化合物7(DDC7)和鼠李糖脂(C5)抗革兰阳性球菌的作用机制。方法分离纯化金黄色葡萄球菌ATCC29213细胞壁并用透射电镜观察。依据新化合物对ATCC29213已知的最低抑菌浓度(MIC)结果,用肉汤二倍稀释法,检测新化合物加入不同浓度细胞壁后对ATCC29213的MIC。结果新化合物DDC7、C5对ATCC29213的MIC为16μg.mL-1。当加入细胞壁浓度≥32μg.mL-1,可以阻断DDC7、C5分别对ATCC29213的抑菌作用。结论糖脂新类化合物抗菌作用部位在革兰阳性球菌细胞壁上。
Aim To study the mechanism of action of new compound rhamnoglycolide-derived compound 7 (DDC7) and rhamnolipid (C5) against Gram-positive cocci. Methods The cell wall of Staphylococcus aureus ATCC29213 was isolated and observed by transmission electron microscopy. Based on the minimum inhibitory concentration (MIC) results of the novel compounds known to ATCC29213, the MICs of the new compounds against ATCC29213 after addition of different concentrations of cell walls were tested using a two-fold dilution of broth. Results The MIC of novel compounds DDC7 and C5 to ATCC29213 was 16 μg.mL-1. When added to the cell wall concentration ≥ 32μg.mL-1, can block DDC7, C5 were ATCC29213 antibacterial effect. Conclusion The antimicrobial activity of the novel glycolipid compounds on the cell wall of Gram-positive cocci.