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采用扫描和透射电镜技术对自然成熟的条纹锯精子、卵子及精子入卵过程进行观察。观察结果显示,其精子由头部、中段和尾部三部分组成:头部主要由细胞核构成,无顶体结构;中段由线粒体、中心粒复合体(近端中心粒和基体)、袖套组成;尾部主要由轴丝组成,外部包裹质膜,轴丝为典型的“9+2”结构。卵子表面分布纵横交错的网纹,均匀分布着大小不一的微小孔,在卵壳的动物极精孔区的中央有一个受精孔。在授精后10 s即可见到精子通过受精孔进入卵子,刺激卵子发生形态变化封闭受精孔,阻止其他精子入卵,60 s可见受精孔完全封闭。
Scanning and transmission electron microscopy (SEM) were used to observe the ovulation process of spermatozoa, eggs and sperm in naturally mature streaks. The results show that the sperm consists of three parts: the head, middle and tail: the head is mainly composed of the nucleus, no acrosome structure; middle by the mitochondria, centriole complex (proximal centriole and matrix), cuff composition; The tail is mainly composed of the axis of the wire, the outer envelope of the plasma membrane, the axis of the wire is a typical “9 +2 ” structure. Ovum surface distribution criss-cross pattern, uniform distribution of tiny holes of different sizes, in the zygote of the animal very fine hole area has a fertilization hole. 10 s after insemination can see sperm into the egg through the fertilization hole to stimulate the ovum morphological changes of closed fertilization hole to prevent other sperm into the egg, 60 s can be seen completely closed hole fertilization.