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目的:探讨强心灵灵浓缩丸的质量标准。方法:采用薄层色谱法鉴别强心灵中人参、黄芪、麦冬,用HPLC法对制剂中葛根素、丹酚酸B进行定量分析。结果:薄层色谱中,供试品溶液色谱在与对照药材及对照品相应位置上出现相同颜色的斑点。在选定的色谱条件下,葛根素进样量在41.4～331.2μg·ml~(-1)范围内与峰面积线性关系良好,r=0.999 7,平均回收率为99.40%;RSD为0.77%(n=6);丹酚酸B进样量在18.02～144.16μg·ml~(-1)范围内与峰面积线性关系良好,r=0.999 9,平均回收率为99.35%;RSD为0.62%(n=6)。结论:所用方法可靠准确,专属性强,可有效控制强心灵的质量。
Objective: To investigate the quality standard of Qiangling Lingling Pills. Methods: The ginseng, Astragalus membranaceus and Radix Ophiopogonis were identified by TLC. The content of puerarin and salvianolic acid B were determined by HPLC. Results: Thin-layer chromatography, the test solution chromatography with the reference substance and reference substance corresponding spots appear the same color. Under the selected chromatographic conditions, the injection volume of puerarin has a good linear relationship with the peak area in the range of 41.4 ~ 331.2μg · ml ~ (-1), r = 0.999 7, the average recovery was 99.40%, RSD was 0.77% (n = 6). The linear range of peak area of salvianolic acid B injection was 18.02 ~ 144.16μg · ml ~ (-1), r = 0.999 9, the average recovery was 99.35%, RSD was 0.62% (n = 6). Conclusion: The method used is reliable, accurate and specific, which can effectively control the quality of strong mind.