本文首次报道了华癸根瘤菌在水稻、小麦、油菜和棉花４种非豆科作物根圈的定殖能力。分别通过盆栽试验并采用外源标记基因（ｌｕｘＡＢ和ｇｕｓＡ）特异性检测技术进行检测，研究发现华癸根瘤菌不但可以在这４种非豆科植物根圈定殖，而且还能进入根内，其数量可达１０３个／ｇ以上。接种华癸根瘤菌对油菜和棉花根系的生长有一定影响，并能显著促进水稻的生长，为扩大华癸根瘤菌的应用范围展示了诱人的前景。 This paper reports for the first time the colonization of Rhizobium japonicum on the rhizosphere of four non-legume crops of rice, wheat, rape and cotton. The results showed that Rhizobium japonicum could not only colonize the rhizosphere of these four non-legume plants but also enter the root, which was tested by pot experiment and using the exogenous marker gene (luxAB and gusA) The number of up to 103 / g or more. Inoculation of R. solani had some effects on the growth of rapeseed and cotton roots, and could significantly promote the growth of rice, showing an attractive prospect for expanding the application range of R. solani.