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目的:研究肾母细胞瘤患儿术前、术后血清蛋白质谱差异,检测术前、术后特异性蛋白质标记物来指导预后。方法:应用表面增强激光解析电离飞行时间质谱技术结合支持向量机分析所有质谱数据并检测肾母细胞瘤患儿术前后血清蛋白质标记物的表达差异。结果:(1)30例术前标本与正常对照组比较:初步过滤筛选得到352个m/z峰,筛出m/z峰值位于6 984.0和6 455.0的蛋白质标志物2个,与正常组比较,患儿组此两峰处的蛋白质低表达。(2)24例手术前后血清蛋白质的比较:术前均呈低表达或不表达;21例根治术患儿术后2周标本高表达19例,低表达2例,3例姑息性切除患儿标本手术前后比较无变化;术后3个月和6个月跟踪复查时血清蛋白质在此两个峰值处无明显改变。(3)患儿术后2周中的19例根治术后与术前比较m/z峰值表达升高,与正常对照组比较表达稍低。结论:肾母细胞瘤特异性血清蛋白质标记物可以用于肾母细胞瘤的快速检测和监测预后,为患儿的预后提供新方法。
Objective: To study preoperative and postoperative serum protein profiles of children with nephroblastoma and to detect preoperative and postoperative specific protein markers to guide the prognosis. Methods: Surface-enhanced laser desorption / ionization time-of-flight mass spectrometry combined with support vector machine (SVM) was used to analyze all mass spectrometry data and detect the difference of serum protein markers before and after treatment of nephroblastoma. Results: (1) Comparison of 30 preoperative specimens with normal control group: 352 m / z peaks were obtained by preliminary filtration and screening, and 2 protein markers with m / z peak at 6 984.0 and 6 455.0 were screened out, compared with the normal group There was a low expression of protein at the two peaks in the pediatric group. (2) The serum protein of 24 cases before and after surgery showed low or no expression before surgery; 21 cases of radical operation 2 weeks after operation, the specimens were highly expressed in 19 cases, low expression in 2 cases and 3 cases of palliative resection in children There was no change in the specimens before and after surgery. Serum protein did not change significantly at these two peaks at 3 months and 6 months after operation. (3) The expression of m / z peak in 19 patients 2 weeks after operation was significantly lower than that in the normal control group after operation. Conclusion: Nephroblastoma specific serum protein markers can be used for the rapid detection and prognosis of nephroblastoma and provide a new method for the prognosis of children.