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食管癌的发生发展与多种癌基因、抑癌基因改变有关,但尚未发现与食管癌有高度相关的基因.我们用mRNA差异显示方法对正常人上皮和中国河南省林县高癌家族的食管组织,进行基因表达的比较,找出了4个差异条带.经RT-PCR鉴定其在正常组织中表达而肿瘤中不表达或低表达.我们进一步用5’-RCAE方法钓取其中一个基因片段的全长cDNA序列,命名为食管癌相关基因-1(esophageal cancer relat-ed gene-1,ECRG-1).该序列经Goldkey软件分析,具有一包含1176 bp的完整阅读框架,编码蛋白包括392个氨基酸,蛋白分子量为45kD.我们选用原核表达系统中的融合蛋白表达载体,以获得ECRG-1基因编码的重组蛋白.
The occurrence and development of esophageal cancer is associated with a variety of oncogene and tumor suppressor gene alterations, but no genes with high correlation with esophageal cancer have been found. We use mRNA differential display methods for normal human epithelium and the high cancer family of esophagus in Lin County, Henan Province, China. Tissues were compared for gene expression and 4 differential bands were identified. RT-PCR was used to identify them in normal tissues but not in tumors or low expression. We further used the 5’-RCAE method to acquire one of the genes. The full-length cDNA sequence of the fragment, named esophageal cancer relat-ed gene-1 (ECRG-1), was analyzed by the Goldkey software and has a complete reading frame of 1176 bp including the encoded protein. 392 amino acids with a molecular weight of 45 kD. We used the fusion protein expression vector in the prokaryotic expression system to obtain the recombinant protein encoded by the ECRG-1 gene.