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目的观察CD36功能肽段YRVRYLAKENITQDPEDH(93-110)对小鼠实验性矽肺纤维化的阻抑作用。方法将小鼠按体重随机分为4组:生理盐水组、SiO2模型组、CD36功能肽段组(93-110)、CD36对照肽段组(208-225)。采用暴露式气管内注入法染尘,分别于第7、第14、第21天处死动物,HE、VG染色进行肺组织病理学形态观察,采用碱裂解法测定肺组织中羟脯氨酸含量。结果染尘7 d,病理检查表明,CD36功能肽段组小鼠肺组织肺泡炎症较SiO2模型组和CD36对照肽段组轻。小鼠羟脯氨酸含量在各组间的差异也没有统计学意义(P>0.05)。在染尘后14 d、21 d时,CD36功能肽段组小鼠肺组织纤维化程度明显轻于SiO2模型组和CD36对照肽段组小鼠,羟脯氨酸含量明显低于SiO2模型组和CD36对照肽段组,差异有统计学意义(P<0.05)。结论CD36功能肽段对小鼠实验性矽肺纤维化具有明显的抑制作用。
Objective To observe the inhibitory effect of CD36 functional peptide YRVRYLAKENITQDPEDH (93-110) on experimental silicotic fibrosis in mice. Methods The mice were randomly divided into 4 groups according to body weight: saline group, SiO2 model group, CD36 functional peptide group (93-110) and CD36 control peptide group (208-225). The exposed endotracheal intratracheal inoculation method was used to infect the dust. Animals were sacrificed on the 7th, 14th and 21st days, respectively. HE and VG staining were used to observe the histopathology of the lung. The contents of hydroxyproline in the lung tissue were determined by alkaline lysis. Results Dyeing for 7 days showed that alveolar inflammation in lung tissue of CD36 functional peptide group was lighter than that of SiO2 model group and CD36 control peptide group. The hydroxyproline content of mice in each group also showed no significant difference (P> 0.05). At 14 d and 21 d after infection, the degree of lung fibrosis in mice with CD36 functional peptide group was significantly lower than that in SiO2 model group and CD36 control peptide group, and the hydroxyproline content was significantly lower than that in SiO2 model group and CD36 control peptide group, the difference was statistically significant (P <0.05). Conclusion CD36 functional peptide has obvious inhibitory effect on experimental silicotic fibrosis in mice.